a_j
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PCR is a necessary part of Sanger sequencing but I would say that it is not possible to sequence with PCR. What u know is that the primer attaches at a certain sequence but have to know this sequence first to design the primer..
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I think u twisted the formula. In ur case u get an unit that means 1/g for a mass and I think the units are wrong. the concentration of a solution is calculated by (c (mg/L) = mass (mg)/V (L)) Hope this helps...