a_j

PCR is a necessary part of Sanger sequencing but I would say that it is not possible to sequence with PCR. What u know is that the primer attaches at a certain sequence but have to know this sequence first to design the primer..

I think u twisted the formula. In ur case u get an unit that means 1/g for a mass and I think the units are wrong. the concentration of a solution is calculated by (c (mg/L) = mass (mg)/V (L)) Hope this helps...