throllen

ok suppose i want to take a short DNA strand like this one 5-gatcctgcta-3 into RNA, and want it to be labeled. So radioactive GTP is used. And supposed if the GTP is radioactived with the additon of 4th phosphate group in the alpha postion, will the resulting RNA be radiolabeled? What about the beta position? I don't get this at all

my prof in genetic class gave everyone this take home question for us to think about: What would happen to the supercoiling of the DNA if the C2 and C3 carbon bond at the sugar backbone is cut? Shouldn't it disrupt the supercoiling in a way that all the base will not stack anymore? This one is a toughie...