Dear CharonY,
Thanks for your reply. I've done quite a bit of work in molecular biology but I just started Western business.
I am doing membrane stripping today and I could see so many different protocols with regard to this.
Having just few questions, please:
1) Some people mention that beta-mercaptoethanol traces may damage antibody (AB) in the future probing
Others seem to be pretty relaxed about this concern. What would be your take on this?
2) I am trying to do 8 (eight) Western gels/membranes at a time. How many of those usually people do?
