whusean

Hi, If you get infected with the X serotype and survive, you will likely become immune to serotype X. Therefore further X infection will unlikely result in clinical symptoms. Serotype Y would be marked with a varying antigenic profile (compared to X), therefore infection with Y will mean that the person will become ill as the immune system has had no prior encounter with Y. The reason why secondary infections are more virulent is because of a process called antibody-dependent enhancement (ADE). Your better of googling ADE because its quite complex, and i cant really be bothered to explain it all. Regards

Hi sorry about the delay. Each LHC-II protein contains 14 chlorophyll molecules.

Hi all Ive done a UV/VIS on a solution containing chlorophyll bound to its protein (LHC-II). Once ive worked out the concentration of chlorophyll via Beers law, does any one know how i can then calculate the protein concentration. I reckon it is prob easy but i cant work it out Thanks

My SDS separating gel contains Tris/SDS and im trying to calculate the conc of this in the gel.... The tris/sds stock solution was made initially from: 6.05 g Tris 20ml of 20% SDS 80 ml water to give a total volume of 100ml. So Tris/SDS was made in this solution. I want to calculate to concentration of Tris/SDS in this solution, so do i have to calculate the tris and SDS concs seperatly or together,,,if you get what i mean. I hope this is clear, any help will be much obliged

I wouldn't say charge is negligible. Granted, size of protein has a larger impact than charge, but its the mass/charge ratio which is taken into consideration.

That is what i thought thanks. I am diluting this buffer so would it be ok to express the conc as X follwing the dilution. E.g. 2.5 X Tris. This would be included in my final year project, and i have been told expressing conc as X is not generally liked.

Can someone help me out with this. Im trying to convert the follwing buffer...4 X Tris, to be expressed as a molarity. This is all the information i have, and is this possible?

Ok i get that...so what happens when the infection could be caused by a number of possible pathogens. How can a specific gene specific to one pathogen be targeted?

Can someone explain exactly how PCR is used to diagnose the presence of a microbial infection. Are primers which are specific for certain microbial genes designed, and if that microbe is present, then that gene will be amplified??

Yeah thats great, much appreciated

Im Just putting it out there, but who thinks melarsoprol is one of the most toxic and dangerous drug treatments currently used in medicine. Nasty, nasty drug but is one of only a few treatments for late stage HAT.

I think i understand it now..thanks alot. Just one more quick question....once i get the conc in mg/ml, it would then need to be converted into mg/100 ml in order to get a % conc value right?

What is the significance if these are produced at CERN. What will this mean for the world of physics??

I'm not following you to be honest...is there any chance of you explaining it again, in a slightly different way. Many thanks

Sorry about that i dont know what happend there. Basically what I said was; Im currently writing my final year dissertation and I need to write up about an SDS page i did. My supervisor told me to write the composition of stacking/seperating gels as concentrations and not volumes. Im having trouble calculating these concentrations, so a bit of help would be much appreciated. I just need to know how to properly write it in my final project. The composition of my stacking gel was: 7ml 30% acrylamide/ 0.8% bisacrylamide 12.5 ml 4X Tris buffer 25.5 ml H2O 10 ul 2% bromophenol Thanks in advance

Hi people<br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); "><br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); ">Im a 3rd year biomedical student currently writing my dissertation. I am confused about how to correctly write the composition of my stacking/separating gel. My supervisor told me all of the components e.g. Tris must be expressed as concentrations and not volumes, but im having trouble working this out.<br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); "><br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); ">The composition of my stacking gel was:<br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); "><br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); ">7 ml 30% Acrylamide / 0.8% Bisacrylamide<br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); ">12.5 ml 4X Tris<br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); ">25.5 ml H20<br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); ">10 ul 2% bromophenol<br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); "><br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); ">Could anyone help me understand the correct way i should express this in my final report. <br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); "><br style="font-family: verdana, geneva, lucida, 'lucida grande', arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(245, 245, 255); ">Thanks