BPLabscience

Yes. And we select for that when growing up our bacterial colonies. We are also using pGLOW, not pGLO

We have only validated LacZ. Is there a way of validating GFP without measuring excitation/emission?

Over the past few months our lab has been attempting to measure expression differences between promoter sequences through transfection. We have a pGLOW plasmid to measure GFP expression on our two sample groups and a pCMV-LacZ control to measure transfection efficiency and number of plasmids per sample. The trouble we have run into is that GFP is expressed only if our LacZ plasmid is not present. We think something about the LacZ trasnfection interferes with pGLOWs transfection, and only LacZ gets transfected into the cells. I have a couple of questions: First: Has anyone come across this problem before? If so, how did you manage to fix it? Second: I find that pGLOW is rarely cited in publications as the experimental plasmid, has anyone had trouble with using pGLOW for dual transfection? Is there a better plasmid system to use to measure the effect of a SNP on gene expression? Any help would be appreciated.