Given the sequence:
TCAGTTCAGCCCTCACCATTGCTCTGCAACTCAGAACAGTTACTACTGAGACTACCCTGAGAAGAGGATG
GTCCTAAACAGAGCTCTGATTCTGGGGGCCCTCACCCTGACCACCATGACAAGCCTCTGTGGAGGTGAAG
ACATTGTGGCGGACCACGTTGGCACTTATGGCACAGACTTCTACCAATCTCATGGTCCTTCTGGCCAGTA
CATCCACCAATTTGATGGAGACGAGGAGTTTTATGTGGACCTGGAGAAGGAAGAGGCTGTCTGGAGGCTG
CCTATGTTTGATAAATTACGTTTTCACCCGCAAGGTGCACTGAGAAACATAGCTATAGCGAAACACAACT
TGGATGTTCTGACAAAACTCTACAACTTTACCCCTGTTATCAATGAGGTTCCAGAGGTGACTGTGTTTTC
CAAGTCTCCCGTGATGCTGAGTCAGCCCAATACCCTCATCTGTCACGTGGACAACATCTTTCCCCCTGTG
ATCAACATCACATGGTTGAGAAACGGGCACTCTGTCACAGAGCATGTTTCTGAGACCAGCTTCCTCCTCA
GAAGTGATTATTCCTACCTCAAGATAAGTTATCTCCCCTTCCGCCCTTCTGATGATGATGTTTATGACTG
CAAAGTGGAGCACTGGGGCCTGGATGAGCCACTGCTCAAACACTGGGAGCCTGAAATTCCAGCCCCTATG
TCAGAGCTGACAGAGACTGTGGTCTGTGCCCTGGGGTTGACCGTGGGCCTCGTGGGCATCGTGGTGGGCA
CTGTCCTCATCACCCGAGGTCTGCGCTCAAGTGGGGCCTCCAGACACCAAGGGCCCTTGTGAGTCACACT
CCAGTAGGAAGGTGTACTGTCCATCTACAAGAACAGAAAAATGGACATACTAGATGACCTGGAACTATTT
TCTGGCCAAGTTCATCACGTACCTTTTCTCCTTCTGCCCTCCTCTGCTCACCTCTTCTCTGGGAAATAGG
ATGCTGTATCACCTCAGAGTTCACATATACCTCAGAGTTCTTGCCCTGACTATGTGATATTTCTTTCTCT
TCTCATCAGTTGCATAACATGGAATTGCTACGGTATTCCACCCAATAACCTAATCTCTAGTGACCCTGAT
CCCATGTCACCACGGAAGCAATAAATCCTTCTTTTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
we have to find...
a.Start codon (red)and Kozak sequence (blue).
b.Stop codon(red)
c.exon boundaries(yellow).
d.reading frame (+1, +2 or +3).
e.polyadenylation signal (not polyA tail)(green).
We we're told to use the NCBI blasting website to help.
If you could help with any of them, please let me know!!
