janu
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improving recominant protein expression
janu replied to janu's topic in Biochemistry and Molecular Biology
protein is human growth hormone and strain is e coli bl21 -
hi all i have cloned 600 bp gene in pet vector in colony pcr i have got positive result ,i have got amplification in that once after expression analysis of protein with IPTG induction i am getting very less amount of expression can anybody suggest me how to improve expression of target recombinant protein thanks in advance