Zoops
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Would ethylene glycol or propylene glycol work as a preservative of daphnia?
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Does anybody know of a good Freshwater Ecology Graduate Program. I would like to skip the masters and go right for a PhD but I can't seem to find what schools I'll benefit the most from for Freshwater Ecology. Any advice will be helpful
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Thank you for helping me out I now just need to get access to this sort of equipment.
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I was wondering if anyone knew of some good freshwater ecology graduate programs. Or where I could find that information.
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Thank you both. If I do freeze them at -80 do I need them in anything such as an alcohol or another medium. Or just freeze the organism by itself. And I'm assuming the temperature is in celsius? Also for the ln2 dreary I was looking online and they only hold for what looks like up to about 2 1/2 months to 3. Is there anything that would store them longer. Also with the RNAlater can that sit at room temp or should it be in a freezer as well. Sorry for more questions.
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My name is Tom I like genetics and freshwater ecology, and I am a zooplankton taxonomist.
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Hey Everyone I was wondering if anyone would be able to help me out. I am going to have access to a large number of Daphnia of varying species this summer and I was going to take subsamples and save them for a later date. I want to eventually be able to use them for genetic sequencing but I will not be able to extract any DNA from the Daphnia right away. I was going to initially store them in 70% ethanol and store them in the freezer, because when I worked in an RNA lab we store precipitated DNA in isopropanol and I think a buffer and then put them in a freezer. I then read online somewhere that storing them in ethanol could skew the results when they were used to run gels. So I was wondering if anyone had any idea of how I could store whole Daphnia until it came time for me to start extracting the DNA and sequencing. It will probably be anywhere from 1 to 2 years before I would actually be able to sequence them. Any advice would be helpful.