dilipsingh

Hello all this was assay i standardized for initial quantification of lipid without extracting them. This assay is cost effective, fast and can be scaled up for large numbe rof samples for initial qunatification of lipid from whole cell. I have studied the reaction mechanism of this assay from literature and it show that vanilin-PA regent selectively reacts with one double bond per molecule of fatty acids irrespective of more than one double bond. Traditionally initial quantification of lipid is, first you have to lyse the cells then isolates lipid which is time consuming,solvent consuming since extraction efficiency is not 100 % so compormising with results. So with assay i can quantify lipid of large number of samples within short span of time.

Hello thank you all for ur suggestions its not about buying the saturated one actually i have standardized a colorimetric assay (sulfo-vanillin phosphoric acid assay) to quantify lipids from whole algal cells without extracting them but the problem is it gives reaction with only unsaturated fatty acids not with saturated one, leading to flaw in this protocol. so what i was thinking to treat standard saturated fatty acids (SFA) to create double bond in it,so this regent will react with double bond and will give positive reaction and fatty acid desaturaes i cant use at high temp along with higher conc. of H2SO4. since i am doing my phd in biodiesel field so it is important to quantify extact amount of lipids in whole cell. what about first halogenation followed by dehydrohalogenation (with alc KOH, elemination reaction) any idea???????/

hi i need a help in organic chemistry regarding a problem,which i googled but unable to find to find solution my problem is"i want to convert saturated fatty acids into unsaturated fatty acid and i was thinking to use alchoalic KOH but it will rahter give saponifiaction reaction, i want to know a simple and easy reaction or any regent which i can use to create unstauration (double bond in any number) in fatty acid which i can carry out in my biotechnology lab,,,since my background is biotechnology not chemistry, so i need a serious suggestion from the member of this group thank you