angelkissesH

How would I regenerate an HPLC column after it has *dried out? * Dried out means, someone else took my column off the HPLC machine for me and didn't replace one of the end fixtures. I didn't realize this until ~2.5 weeks later. Air hasn't been pumped through the column, but I'm getting unusual UV traces (High absorbance in irreproducible areas). Does anyone have any suggestions?

Hello, I'm trying to convert a kcat/km obtained from a journal article from s-1*uM-1 to s-1*M-1. kcat = .55 s-1 Km = 71.7 uM kcat/km = .008 s-1*M-1 I'm assuming that I just need to convert the 71.7 uM into M (.0000717M) and then divide to get the value 7670 s-1*M-1 However, and older, much respected labmate claims the value should be 4.8 E5 in his notes. Am I doing my math wrong? Thanks!!! Merged post follows: Consecutive posts mergedI turns out they were converting it to min-1 * M-1 not s-1* M-1. Thanks!!

I beleive I've read somewhere (lecture notes from the internet so not exactly from a good source) that NADP+ doesn't absorb at 340nm. Based on the buffer solution, could it be possible that some of you NADPH may have converted to NADP+ thus decreasing the conc of NADPH (and the extinction coefficient)?