pensive

no one helped no one helped

The context we were given for haploid was a fungus called Aspergillus nidulans but I think that it has a diploid phase as well. Crossing technique would be helped if explained!

Hi, I was given a workshop on how to do a two point test cross. But the lecturer didn't go over three point. I went off to do it by myself and spent hours trying to work. Long story short, I got the progeny for cross like this. V (vermillion) cv (crossveinless wings) (ct (cut wing edges) --> mutant alleles Wily type--> + (red eyes) +(cross veined) + ( normal wings) v ct cv/ + + + (arbitrarily ordered female) x V ct cv/ v ct cv (male) I got 8 progeny from double and single crosses. v + + = 580 +cv ct = 592 Those were the parental V cv +=45 ++ct=40 v ct cv=89 +++=94 single crosses v+ct=3 +cv+=5 double crosses and total progeny 1448 Anyway, I worked out that the ct allele should have been in the middle in all of the above genotypes. by reordering the parental chromosome. But the bit I'm stuck on is calculating the map units. The alleles in the female were thus arranged like this v++/+ct cv in this exact order. So I was told to look for the V-ct loci and then work out the RF for this, but how do I do that? I need to do this for ct-cv and v-cv loci as well. Please could someone help me. I really,really,really stuck and annoyed because I want to work this out!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! Cheers

Hello everyone I was wondering something. During our lectures I have learned about the concept of genetic mapping but now really how it is achieved. In outline, how is such a thing done in diploid and haploid organism. I'm assuming that in haploids, maybe someone sort of test cross is done where a known homozygote and heterozygote (if that is possible to have in haploid) are crossed and the progeny are observed. But, I'm not 100% sure how a diploid would be crossed. I did try looking around but didn't get a straight answers. Obviously, there would be a known homo and hetrozygote again. But the procedure I don't know. Cheers guys for the useful information!