soconfused

Can anyone help me getting full text of this article, please? Thank you a bunch! http://www.ncbi.nlm.nih.gov/entrez/query.f...5&dopt=Citation Biochemistry. 1997 Feb 11;36(6):1467-78. The divergent 5' termini of the alpha human folate receptor (hFR) mRNAs originate from two tissue-specific promoters and alternative splicing: characterization of the alpha hFR gene structure. Elwood PC, Nachmanoff K, Saikawa Y, Page ST, Pacheco P, Roberts S, Chung KN.

The stock i bought is defined in 12% and not in molarity. Any ideas?

What is the unit of 10,000 and 1,000,000 in this case? mL or L? Should i not consider the densities of both solute and solvent when both are in the liquid phase? Besides the one i use is Sodium hypochlorite (NaOCl), with a stock concentration of 12% . Will you please show me how to calculate 10,000ppm hypochlorite from a 12% stock? It just says the vapor density and not the density for the solution. Do you mean; 1 p.p.m. = (mol.mass of solvent)/(mol.mass of solute) x mG/L? It seems like you forget something before mG/L, because i don't understand the formula. Thanks.

I have to make a solution of 10,000ppm hypochlorite, since 1ppm is like 1mg/l for a solute in water solution. Does this mean that i have to add 10,000mg hypochlorite in 1liter water? If hypochlorite is in a liquid form, should i take into account its density and from that to find the volume i add to the water to get a totol of 1 litre? Besides; Is 1ppm the same like 1mg/l ? Or does it depend in other factors like both the densities of the solute and solvent? Thanks for any inputs.

Uh, i never thought that they are different things. I would test up or down regulation of gene expression. Which one should i use? THanks.

good links and info of RT- PCR? appreciate for any inputs.

hi guys! what types of cells is a mammalian cell? i know this is maybe a "horrible" question, but i find it hard knowing the different cells under this category cell. i have looked in the net, but don't find any site where they explain about this type of cell. so hope for some ideas. thanks!

hi guys! i have difficulty in understanding this sentence, since English is not my language. here it is : "The elution volume (Ve) is the volume of buffer before which the peak of protein elutes." hallo! yes, i don't get it. does it mean Ve of that protein is the volume of the buffer before the UV-absorbance of that protein or does it mean that the volume of the buffer to the middle of the peak? p.s! UV-absorbance and peak are different things here. thanks for helping!

wrong forum!

thanks very much for your sharing, Frostrunner!

chromatography: we have to collect the fractions from the column. i wonder what is the difference between; flowthrough fraction, wash fraction and eluate fraction? i think i know what it means with eluate fraction, but what about the other two? hope for replies! thanks a bunch!

thanks alot Dave and Woxor!

hi skye! are you sure column cross-sectional area (cm2) has this formula? thanks!

hi guys! i am doing the chromatography. in scaling up i have to increase my volumetric flow rate but maintain my linear flow rate. my problem is i don't know how to calculate the column cross sectional area (cm2). i have asked the people in the math forum, but it seems like we get nowhere, so i hope you guys who work with this area can tell me how i can calculate it when the radius of the column is 2cm and the height is 5cm. Linear flow rate (cm/hr) = volumetric flow rate (cm3/hr) / column cross-sectional area (cm2) thanks a bunch!

so what is cross-sectional area?