question

I want to isolate DNA from the bacterias (both Gram positive and negative bacterias) in drinking water. Do you have any protocols which is good for this purpose? THank you very much!

I have made a correlation between group A and B and the pearsson coefficient constant, r, is at 0.97. The values are expression levels and hence they are non-parametric. I wonder how can i calculate the p-value of this correlation between group A and B? I know how to calculate the p-value for the difference between group A and B, but not for the correlation between group A and B. Thanks for any inputs!

I am looking for any references talking about WESTERN BLOTTING, but i hardly find any in pubmed. Could it be that WESTERN BLOTTING is an old technique that is why old articles are deleted or not in use? Do you have any good ones that i can read? I am not interested in some of the unknown websites talking about this topic, since i don't know if they are right about what they are saying. I need articles with references! Thank you!

How can i make a histodiagram in Excel by using Microsoft Windows Version 2002? Or how can i make it, which softwares? Thank you!

Dear all, I have two independent and non normalized samples where one sample has n=30 and the other has n= 1. Due to both samples don't have a normality distribution so i have to use non-parametric methods. I plan using Mann-Whitney Test, but then it needs that both samples have to be larger than n=5 or both are lesser than n=5. In my case then i have n=30 and the other is n=1. What kind of statistical methods should i use to get a reliable answer? I thank you alot! Hope for a quick answer!

I have searched and searched till my eyes fall out, but can not find any recipes for making these solutions. Anyone can help me out? 1mM tributylphosphine 1%SDS 10 M Urea

These are the pituitary gland's proteins i need their sizes infos. 1. B actins 2. Folate receptor alpha subunits do you have any ideas about them?

Where and how can i find sizes of pituitary glands' proteins? When i use Entrez or other bioinformatic sites, it only has human proteins and not specifically human pituitary glands' proteins. Hope for ideas. Thank you.

I am facing a problem where i am not sure if a specific protein like for example pituitary folate receptor alpha subunit has the same function, size and structure in any locations of a human body. as i know a gene can have different start and end signal which means a transcript will end in different products then how come a specific pituitary protein will be identical to other places? Any ideas are greatly appreciated.

I have planned to use 18S rRNA as the normalizer in RT-qPCR for validation of microarray results, do i have to use this same normalizer in my microarray assay? Thank you.

Hi! I already sent you my email address. It will be fine for me if you can put the links here or send me the mail. Thanks!

Aww, someone adviced me to use U-test mann-Whitney, but i don't know if it is the best. What do you think about it? I am testing for the fold difference of some gene expressions between the treated samples (pituitary tumour samples) relative to the controll samples (pituitary normal samples). As i know the experiment must be non-parametric, since the populations don't have normal distributions. Or is it parametric?

You can consider my problem as this: I have two groups. Group A gets treatment while gropu B is the controll where it does not get treatment. In A i have 50 samples/observations (n=50) and in B i have 5 samples/observations (n=5). So what kind of tests should i use to compare group A versus group B? Hope for your inputs and ideas! Thank you.

I am stucked... I would like to read these articles, but they need subscription. Is anyone out there that can give me a full text of them? It will be of very great help for me. http://www.eje-online.org/cgi/content/abstract/153/1/143 http://www.ncbi.nlm.nih.gov/entrez/query.f...864&query_hl=11 Thanks!

Okey, i am confused about this matter. I have two different samples. One is 5 untreated/control samples (same kind) and the other is a 50 treated samples (same kind). I wonder what kind of statistic methods i can use to compare the results between them? I don't think that i can use ANOVA, since they need at least 3 different samples. Besides i can not use T-test or Z-test either, since the former needs both samples to be at a small number n<30, while the latter needs both samples to be large n>30. My samples is n=5 (controll samples) and n=50 (treated samples). Hope for inputs. Thank you!