question

hello everybody!

what does it mean when a gel you use in gel filtration has a "cut off 2.000 daltons" ? i have looked for the answer, but can't find it.

hopes for replies!

many thanks!

so it must be the high of the column.

thanks!

is bed length the heigh of the column or is that the heigh of the matrix/gel?

i don't know where to put this question, but since it is about proteins then i guess here is the place

thanks!

hi skye!

what do you mean? the DNAase i bought was quantified in Units and i have to make a 2mg/ml solution from that stock. hope that you and the others can help me out!

thanks alot!

hello guys!

I need to prepare a 2 mg/mL DNAase solution. the DNAase stock has a concentration of 2U/ml. how can i make a 2mg/ml from a 2U/ml solution? this is quite hard to figure out

hope very much for replies!

thanks for helping!

hi guys!

i am new in this field, so i hope that "mature" people can show me the way. thanks in advance!

with fusion in frame does it mean that the nucleotide sequences of both DNA fragments i fuse with each other have to act as independent triplet unit? in this way we can maintain the polypeptide of both DNA fragments? in other words that their nucleotides don't come together to make the triplet codons from the start to the end no matter if the fusion is a C, N or randomly (please see example 1, below).

for instance if the nucleotides of DNA fragment 1 is k's and it is a plasmid and nucleotides of DNA fragment 2 is x's. i insert 2 into 1. should i before the fusion have to make sure that the DNA fragments i fuse have enough nucleotides each to make their own triplets codon?:

example 1: randomly fusion where i insert DNA fragment 2 in the middle of the plasmid:

....kkk kkk kkk xxx xxx xxx xxx kkk kkk kkk....

here we get inframe from the start to the end. is this the ideal fusion? we can see here that the nucleotide sequences of both fragments DON'T COME TOGETHER TO MAKE THE TRIPLET CODONS. they act as independent units. the polypeptides of both fragments are maintained.

example2: in this case i have used a fragment which does not have enough nucleotides to make the triplet codons for its own. that is why it come with the nucleotides of the plasmid to make the triplet codons. AND THIS IS A FUSION WE SHOULD AVOID, RIGHT? because the polypeptides of fragment 2 is changed, so do the plasmid.

...kkk kkk kkk xxx xxx xxk kkk kkk....

i try to shorten this down, but afraid that none will understand my point, so please be patient and read

thanks alot!

if i insert lacZ into a gene and the translation gives a hybrid product with the sequences of both lacZ and this gene. i wonder if lacZ can still work and give blue colonies? and what about the function of this other gene?

wait so much for replies.

thanks so much in advance!