Dear friends
In my research i need to isolated DCs in popliteal lymph-nodes and then culture them by LPS and then IL12 assay by Elisa.
I used Anti CD11c magnetic beads and autoMACS to isolate DCs.
My Elisa results shows even in culture of DCs with No Ag we can detect high level of IL12 production!
Is it possible it be for magnetic beads??
any recommendation? Using cell sorter is not practical in this case because cell number is very low.
hope to hear you soon.
Thank you very much
Masoud Akbari
Nagasaki University
Japan
