jmjabt

Basically, I switched to BG11 from the UTEX, and they grew from the tiny amount of innoculum I had left after all else failed! I increased light from 2000 lux to 5000 over a couple of weeks as the cultures grew. The project is over now so the cultures have been taken down, but they did grow very well on home made BG11.

Hi, Thanks for the comments. The medium is UTEX as advised by the Culture Collection of Algae and Protozoa. They are no longer in the window and are in a stable temperature of around 28, on 16 hour day and 8 hour night. Based on the advice I will put them a little further from the bulb to give around 3000 lux in the centre of the vessels. These are early in the scale up process and as such are only 120 ml in volume, so light penetration isn't an issue. Upon swirling, there are some 'flakes' for want of a better word, but I cant see any filaments. I have got the paper you mentioned above, but my alga are not in this order, and it seems as it mentions that some species require low light (500 lux) even in the same order, it is hard to know what conditions my species require!! I am still trawling the web for free access literature... I was led to believe I should have dense cultures after 4 weeks to go to the next scale up stage, but this is not the case, hence my worry...! Cheers all, Jack

Hello, I am currently trying to start a series of trials using Oscillatoria tenuis, Anabaena circinalis and Planktothrix aghardiulture the. I have obtained my cutures from CCAP in Cambridge, and am starting to try to culture them. However, I can find very little information on-line on culture techniques, advice, etc. Three weeks ago, I inoculated the cultures at a ratio of 1:10 with UTEX medium as advised by CCAP. I was not sure on light levels so I put them in a slightly shaded window with very gentle aeration. After two weeks I placed them on a light bench with around 5000 lux of standard white fluorescent light (this as the only value I could find on-line). I have since removed the aeration in case this was having an effect. I now have very slightly green water, a little cliudy, but no major growth. I was advised that after 4 weeks I should inoculate again at 1:10 when there was a dense culture. I am nearly at that point and I cant't see any filaments or anything of the sort. I really need someone's help as this is an expensive project, and they are expensive cultures, and I cant get them to do anything... If required I can add pictures. Thanks in advance, Jack Like ThisQuote MultiQuote Edit <br class="Apple-interchange-newline">