rnn

hi! All I am analyzing a transcription factor for its domains, I want to test if one motif about 12 residues long helps in DNA binding directly by making contacts with DNA or by affecting the the DNA contacting domains. Please let me know if there is a way to differentiate between these two possible functions. And if there is an example in literature of any such similar instance please let me know. Any information/input -much appreciated thanks a lot Rnn

thanks will try that, my mac is intel and OSX so hopefully there should not be a problem doesn't seem to be working on my machine, put the folder in the application folder none of the programs get launched any ideas??

yes! I would get the mutated area sequenced and then use seqman to assemble the sequences do you have any suggestion any program that can assemble and align 4 sequences approx a 1-2 kb each would be good for me. thanks

hi! All I was using an old mac and all was well , then I moved to this new intel mac and I cannot run seqman and editseq which is rather important program for my work. I mostly do site directed mutagenesis and used these programs for sequence alignment to check for mutation, IF YOU GUYS KNOW ANY ALTERNATIVE TO THIS PLEASE LET ME KNOW THANKS RN