Mark Ian

Thanks, i just might read that book. The field of science I want to work in (protein design) deals with quantum mechanics. All the books I've read about seem to be at post graduate level, if you can suggest a book on quantum mechanics that is easier to digest, that'd be great! (or anyone)

I have read the wikipedia article on Qubits in which is stated that the possible states a qubit can take can be represented as a position in a bloch sphere. This sphere, a visual representation of the superposition basis state, should be able to hold more than just 3 bits of information. Depending on how accurately the position can be measured, I would assume that a qubit could hold much more information than a mere 3 bites. Furthermore there is a table right under the subtopic physical representation, each point under 'Information support' being a possible bite (giving you at least 13 bit). Plus the probability amplitudes can be expressed as complex numbers increasing the information capacity by a factor or two. I do not understand this.

the thing I can't get over is that a qubit supposedly is so different to a normal bit. The only real difference I see is that qubits have 3 states and a bit has two states of means of storing information. The more trivial quality of quantum computing I see is that its so much smaller than the conventional computer transistor- or is there more to it?

yea, it does work, I just find it more convenient pressing F5. When I run the syntax check though, scite is able to find py_compile.

Hi, Im learning python in the moment. I'm currently using Scite to edit my scirpts, but when I execute the code over Scite using the 'F5' button I get the following error message: >pythonw -u "bottle.py" >The system cannot find the file specified. I'm geussing either scite itself or windows doesnt have the pathway for the python directory... but how do you change it??? Thanks in advance, Mark

thanks, but there aren't

Haha, I do the final volume mistake allot too. As I'm sure you are aware of, the reason you are diluting the stuff is so that you can compare it more easily to other strains. If you were to take only one colony and compare it with another at similar concentrations, you risk of growing too many or to little bacteria for comparison- plus its more convincing. I see why this can be a little confusing: I would think the exponential growth of bacteria would, given enough time, overpower the small starting number of cells used. Try typing in numbers into your calculator: n^t; while 'n' would be the amount of starting bacteria and 't' would be the time that has past. This would sorta mimic exponential growth. For example if you compare growths with 2 cells and 5 time units: 2^5=32 AND with 4 cells and 10 time units: 4^5= 1024 there will always be a significant difference between outcome, given the same time past, and starting amount. You might find the Wikipedia article about Bacterial Growth helpful to understand the concept, especially the different phases; complicated stuff.

Hi, I'm very interested in Protein Design and would like to read some Papers by Brian Kuhlman, the guy is related to the rosetta@home project and the Foldit videogame. Check this link and see if you have a subscription for the paper THANKS! : http://www.sciencedirect.com/science/article/pii/S1074552112000798[b] [/b] as you can take from the address, you would need a subscription at sciencedirect (which sadly my university does not provide). thanks in advance!

this helped me allot. http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cloning_troubleshooting_guide.asp#.UDJUnXU0WSo If you're using electroporation be sure to keep everything on ice, and quickly getting you're bacteria on medium (they literally are in shock). Remember that every second you take will reduce the competence of your bacteria, so work fast. I prefer having two samples, with different ratios of vector to insert (1:3 and 1:7), it may be that the samples with higher concentration will explode, but if it works it usually yields more colonies. Also, since your insert isn't too long you can try amplifying it out instead of the gel extraction. Gel extraction can result in low yield of insert. Sometimes the bacteria simply do not grow well, and you have to work with what you get. I would just pick a few colonies and then digest with your enzyme to see if the insert is there or not.

Cool story stonecat. A combination that I can think of off of the top of my head are MAOI inhibitors, even though this is usually more drug related than food related: the MAOI inhibitor is in a herbal drug, if then combined with specific foods, which require monoamine oxidase for break down, it is toxic. Since many edible plants hold alkaloids, proteins and stuff that effect human anabolic pathways I wouldn't doubt there being a few odd food combinations out there.