wonder

thanks badchad! but the CDS is that the nucleotide sequence of the mature mRNA or premature mRNA? BWT i am a beginner and really want to learn and use the tools in bioinformatic. do you know any place or books which is simple but quite useful? thanks!

where can we get the full length of amino acid sequences? i have very little bioinformatic background so please the more you explain the better it would be. thank you!

thanks!

thanks daisy! i will wait

hi guys! i am doing phage titrering but have difficulty in chosen the right values. please give me some ideas. thanks in advance! here is what we did: dilute phages 5X with dilution buffer. add 1ul of the diluted phage to 200ul of the XL1-Blue overnight culture. Add 2ml melted top agar. Quickly pour onto a plate. the day after count pfu/ml. pfu/ml: (number of plaque*dilution factor*10^3ul/ml )/ul of diluted phage plated. my problem is: what is the dilution factor? is that factor 5 or should i also take 200ul of the XL1-Blue cells and 2ml melted top agar in consideration? (practically it seemed like the phages is diluted more than 5X.) what is the "ul of diluted phage plated"? is that the 1ul diluted phage i added to the other volumes or is that the total volume (1ul phage+200ul XL1-Blue cells+2ml top agar)? thanks!

hi daisy and bluestone, we run the mRNA in the agarose gel to test for the its' stability. p.s in denaturing condition will the mRNA lose its' native configuration? i have not heard about it, hope you will explain. thanks!

hi daisy! it is southern hybridisation. so you think we shouldn't calculate, but optimizing? how can i optimimize? thanks again!

should i add EtBr in the gel solution, when i run the mRNA? thanks!

hi guys! how can i determine the hybridisation's temperature empirically? is there a website for doing this or any tricks? hopes for replies! thanks alot!