Hi I was wondering could anyone help me with a quick dilution question,
When processing sputum in a micro lab dilutions are made to pull out significant microorganisms that are present in high quantities and basically by-pass the normal flora. So basically this is the dilution made:
- using a 10ul loop homogenised sputum is diluted into 5ml of sterile water. ( a 10^-3 dilution?)
- Then a 10ul loop of the 1=^-3 dilution is inoculated from the 5ml bijou onto each agar plate.
- 1 colony then represents 10^5 CFU/ml
My lecturer has asked us to basically explain how we have ended up with 1 colony then represents 10^5 CFU/ml. I have tried but cant really understand how to go about it, can anyone help?
Thanks!
