While doing a simple experiment with different pH levels and catalase, I came across something really peculiar. For some reason when I add sodium hydroxide to hydrogen peroxide and add my catalase (chicken liver) I find that the pH level of 10 is releasing more oxygen than the pH level of seven. I originally thought that when anything over 7.5 would denature the catalase, but according to my data that didn't happen. Is sodium hydroxide some sort of inhibitor for catalase or did I make a mistake? O we used an o2 sensor that was calibrated perfectly before the experiment so there was no error in that. Anybody have any idea?
