M.T. Ardah
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Benzamidine Sepharose and Thrombin
M.T. Ardah replied to M.T. Ardah's topic in Biochemistry and Molecular Biology
Thax, Actually I am using in tubes not columns!! and what is pABA?? -
Hi All, I am using Benzamidine sepharose beads to get rid of thrombin after GST tag cleavage. Afer I added the beads to the protein solution which contains Thrombin I took samples from the beads and the supernatant to check the purity of my protein in SDS and found that most of my protein bound to the beads. Any one have an aidea how to avoid this problem. Regards,