Bluefunk

Freeze-thawing dNTPs can be problematic. I generally freeze and thaw no more than a couple of times before discarding. Small aliquots are the best way. Diluting the NTPs is fine, we use TE buffer to dilute. But basically, yes. the bought dNTP needs to be diluted and stored in small quantities,

Hi, I'm just finishing my PhD in Biochemistry at an english university and I've thinking of applying for post doc positions in the states. I'm just wondering how the work ethic/pay differs on different sides of the atlantic. Postdocs in my group are expected to work Mon - fri 9 - 5/6 obviously working later or working the odd weekend when the experiment requires. BBSRC post doc salary starts at approx £27, 000 ($50,000) but i've heard horror stories of people working 10 hour days 6 days a week in the states and the amount of holiday you get being minimal. any ideas?