SSHan

When Cu2SO4·5H2O is heated, the deep blue color of the hydrate changes to a white color as the water evaporates and the hydrate becomes anhydrous. Can anyone please explain to me why this happens? Thank you! Edit: In addition, I notice that there are five molecules of water per formula unit of Copper (II) Sulfate. I know that four of them are attached to the Cu by coordinate covalent bonds, and one is bonded to the sulfate ion. In addition to that last question, how would a water molecule bond to that sulfate ion? Again, please and thank you!

I'm running a slab gel electrophoresis project for a Chemistry class I'm taking, but for some reason, I cannot correctly see the banding patterns of my experiment for some reason. I have followed the instructions provided by an expert in the field, but I must be doing something wrong, on account of the fact that rather than getting a proper banding pattern when viewing the slabs under a UV box, I get odd streaks on the reused agarose slab, and nada on the newer one (See attached picture). My question: How does one properly use the SYBR green to get the banding patterns. I obviously am doing something wrong. If you need to know what I did, then here it is. After I ran the gels through the electrophoresis process, I submerged the slabs in 500 mL of trisborate buffer. Then, I dropped, using a microliter pipette, 10 µL of SYBR Green over top of the agarose gels (This may be where I made that msitake). Then I let the gels soak for thirty minutes, before setting them up on the UV Box. Thank You and Feel Free to Ask Questions for any Clarification.

Err...no comment...

Correct. I probably should have reworded it better.

Here's a simple one if you think about it. Ignore any misinformation I might have given about the World Cup itself. It's the year 2002, and the Korea-Japan World Cup is just starting. At the first match between France and Senegal, the players of both teams, as well as two referees, lined up to hear the national anthem being played. All players stood at full attention, as well as the cheering fans in respect to the country. All had near-perfect postures, and if you were standing amongst the fans or on the field, you might believe that all of the occupants of the stadium were standing straight up. However, that would be impossible to be true. In fact, one could say that at most one person would have been standing straight up in the entire stadium. Why?

I'm just curious, are there any substances that are: A) Flourescent B) Cannot be separated at the molecular level by any means aside from capillary electrophoresis. The first is optional; the second is a must. Thanks!

Here's one that a friend of mine made: Long long ago in the heart of Europe, there was a nation called Babblenesia, which had many civil wars due to the varying views of its citizens. One day, due to this war, the country of Bablenesia split into two countries. The Southern country was famous for its vast wealth in gold, which they called "yuri" and kept in storage buildings they called "noles." Because of this, many cities of trade popped up in the southern country, and they eventually adopted the English language. The citizens called their country "Yurination", or "Nation of gold." The northern country, on the other hand, became a strict spartan country, with strong armies. They kept their original language, and stayed secluded from the outer world. The northern country's citizens called their country "Yugoslavia", or "Nation of Silver," due to the numerous amounts of Silver deposits there. One wintry day, Yugoslavia attacked Yurination. Yurination, having a small and weak army, was easily taken over. What used to be Yurination, was now Yugoslavia. Feeling pity for the powerless Yurinationians, Yugoslavia allowed the Yurinationian citizens keep their precious yuri noles. So in Yugoslavia, even to today, you can find a bit of Yurination in every yurinole.

I know this may seem like a bit of a dumb question, but which units are Capillary Length and Molecular Separation measured in, regarding Cappilary Zone Electrophoresis? Full names instead of abbreviations would be appreciated if possible.