CharonY

You would change pH but you would have no idea what the buffering capacity of your medium is. Again, a typical buffer systems consists of a weak base/acid and its conjugate acid/base. A citrate buffer could work (though it has some issues) and would be a mix of citric acid and e.g. sodium citrate (refer to the Handerson-Hasselbalch equation for details). Just adding a strong base such as NaOH would raise pH, but you have no clue how stable it is once you add bacteria. I think it is one of the cases where some microbiologists cut corners and disregard basic chemical principles. If your bacteria happens to be E. coli or something related I would recommend looking into M9 medium.

I am not quite clear what the overall points are here, especially as timo pointed out that direct comparisons do not make a lot of sense. However, in this context it should be noted that Germany electricity costs are generally higher than US/Canada even before renewable energies were relevant. The largest part has always been coal. Germany harbored anti-nuclear sentiments at least since the 80s. I do agree that generally there is a lot of nonsense and misinformation about nuclear power in German public opinion, but it does not seem that that are massive changes (which, again is tricky to figure out). A cursory comparison of average household cost per kW (excluding taxes) seems to indicated 0.142 average cost in the EU (2014). Germany is slightly above with 0.144. UK, Ireland, Spain, Belgium, Italy have higher costs. While the newer energies have affected direct cost, it is non-trivial to figuring out total cost (e.g. as maintenance costs could be vastly different, to give one example). Either way, it does not seem that Germany is massively different compared to the others. Take a look at Ausrtia, for example. No nuclear, about 13% fossil fuels and 78% renewables (mostly water). Overall lower prices than Germany. Again, an indicator that such superficial comparisons actually tell very little. There are conflicting studies regarding costs, but those that are not from energy companies indicate moderate cost increase. Ultimately the decision of energy use in a given country will depend a lot on how strong the decision is influenced by environmental lobbying, the power distribution of existing energy providers and their respective ability to influence legislature. In Germany the fossil industry tends to have the upper hand, for example, and local protests can influence decision making. In France the nuclear lobby is quite strong and decisions are more central (to provide a very rough and, presumably, mostly inaccurate narrative)

It is not my area of expertise, but over the years I have heard a number of engineering talks about a variety of carbon capture strategies and systems. One common thing I noticed is that overall net efficiency (especially when adjusted for cost) tend to be much lower than using certain plant systems, especially those with extensive root networks. It is funny as the respective talks (engineered solutions vs analysis of carbon capture efficiency of different plants) tend to be in different conferences, though it would make sense to mix up engineers with ecologists in this case (or at least more than I have seen so far).

The preparation of the buffer depends on the system. Often it is the weak acid (or base) that is titrated with the conjugate base (acid). Others, like Tris are titrated against HCl. You would have to look up for each system you are interested in. Note that most bacteria have a relative narrow optimum and do not grow on a wide range. How you set up the assay is entirely up to you and the bacterium. You could measure detailed growth kinetics (i.e. growth curves) which require liquid broth or you could simply estimate colony size on plates. You could measure generation time or you could have yes/no answers. There is no universal test for everything and you would have to read up what makes most sense (i.e. search lit for the bacterium in question and pH resistance, for example). For the last part you have to understand how molarities are calculated and how percentages work. That is rather fundamental and I advise you really to understand that rather than parrot it from somewhere. If you do not grasp the concept you will be lost. Just to provide a hint: 1 gram of solute in 100 ml total volume equals 1 m/v %. I.e. it is the ratio of the weight with volume.

LB is not a terribly good medium to manipulate pH. In addition, the nutrient levels are not very defined making interpretation a bit trickier. Typically a minimal medium is the best (if more cumbersome) way to conduct theses tests, in which you substitute your buffer system so that you can control pH and buffer capacity. The problem, of course, is that different buffers can affect bacterial growth independent of pH (some may utilize citrate, for instance). What can be used as buffers, depend to some degree on your organism. Some bacteria are sensitive to high phosphate levels, for example. Generally, for a given pH, choose a buffer that is a) not harmful to your bacterium, b) is not utilized as nutrient c) ~ 1 unit within the pka of the buffer system d) has sufficient buffer capacity. The latter depends on whether it is expected that your bacterium produces significant amount of acids, for example. Typical buffers used are: cabonate, MOPS, HEPES, Tris, Ethanolamine, CAPS, ACES, for example

This is because it is assumed that companies that offer paid internship value interns more. However, it can differ from industry to industry and in some it is more about e.g. access to networks (as iNow mentioned).

Funnily I often get sent those tissues sent for analyses (mostly RNA or proteins). Unfortunately many collaborators do not set up the tox test properly, resulting in results that are hard to interpret.

This is not really my primary area of expertise, but what I can tell you is that the different isotypes have different properties. From memory, in antibody engineering IgG3 is normally not used. Furthermore, IgG1 and 3 typically (but not exclusively) act against protein antigens, 2 more against carbohydrates and 4 is involved in chronic responses. Half-life is similar between 1 and 2 and drops significantly for 3 and 4. Abundance is highest for 1 (~60%) followed by 2, 3 and 4 in that order. 1 and 3 require additional effector functions, 2 mostly not (which makes it attractive, I do not recall details for 4. Mind you, this is more from the viewpoint of antibody engineering rather than vaccination, though. Just due to abundance my guess (with emphasis on guess) is that 1 and 2 would contribute the most, covering proteins and carbohydrates as antigens (including the potential to act in concert).

Well, recent studies suggest that empathy has developed at least in social mammals quite early on. One assumption is that this is necessary to form any social structure (i.e. to be able to identify yourself to some degree with someone else). As in many species social behaviour has a number of advantages, it can be assumed that elements such as neuronal elements involved in evoking empathy are under positive selection in many cases. The development of morality is, potentially an abstraction of these biological foundations.

These remarks seem to indicate that Trump was not addressing the smugglers, but rather illegal immigrants. Of course it does not necessarily mean that he thinks Mexico is full of rapists. Just those that cross the borders, I assume.

Take a look at this book . There is a description and comparison of some older microtomes including the Harris. Not a detailed manual, but provides principles and basic operations (around p.144).

In fact, pretty much impossible. Although some advances have made the fabrication of movable parts possible. For nanoparticles much more info is available. However, most is based on in vitron analyses and it is not clear if the performance is the same in vivo. Plus there are some unknowns with regards to toxicity of these materials. So while the research is accelerating, there are still things to figure out. At least as additives the use could be already quite advanced (considering that nanomaterials have been in household products for quite a while for non-medical use) but the high-end stuff (highly specific drug delivery) is still a bit out

If you envision small automatons in the nm scale, they do not exist. Complex nanoparticles, however, are sometimes referred to as nanobots. Mostly because it sounds more advanced.

Dawkin's book are written for laypersons, which is fine for high school level. These books do not have the level of details that would change within a few years.

Well, my interests would be more in the area of molecular toxicity, which involves molecular (e.g. transcriptomic/proteomic) analyses of toxic effects. I am not sure how feasible such a setup in this context would be, though.

Never. Xrays are way shorter than visible light. While you can use false colours the idea of using it for colour imaging is meaningless. Same goes for ultrasound, they are sound pressure waves. I have no idea why you want to render it in colour. They are getting better for 3D imaging, though. For nanobots there is no real concept (unless very simple structures that have been termed nanobots, but they are often not what the popular lit. describes as such. With 3D printed organs, again, it depends on what target organ you are thinking of and how big the involvement of 3D printing is. A complete functional organ such as the heart completely 3D printed is easily way more than 50 years away. A simple 3D structure with little internal complexity such as outer ear (basically 3D printed scaffold seeded with cells) could be within 15. The rest is anyone's guess. There are plenty of those around. Usually specialists but also larger joint practices have at least x-ray but often also ultrasound (especially gynecologists) around. It is not that common for family doctors, but then you have to keep in mind that they are often there to send you to the right specialist. When you talk about different rooms, it is about efficiency. It does make sense to have a dedicated X-ray room that can be used by everyone rather than buying multiple instruments that are only being used for a few minutes at a time.

That would be the wise thing to look out for. Especially things certain businesses are notorious for hiring unpaid (or minimally paid) interns solely as cheap interim workers. Obviously, these should be avoided.

Actually the selection is a bit odd as it mixes things that already exist with things that are still in development. Phage therapy has been proposed as treatment of bacterial infections, though their use for cancer treatment is not as advanced. Ten years would be a very optimistic view (IMO). Targeted drug delivery is partially there for certain areas but typically not at the desired "breakthrough"-specificity. Nanobots are even more sci-fiy, as mentioned. Lasers and various means of heat treatment are already in use for tumors and cancers, for example. Handheld ultrasound scanner can be bought, they are not that expensive, anymore. Same about handheld x-ray scanners (for quite a long time, depending on the definition of handheld), though they tend to be on the bulky side. The latter typically not used for medical purposes, though.

From which perspective? For the most part it depends on the business it is their decision to offer payment. There are various consideration beside cost that may determine whether internship at a given company is paid or or not. For example, in areas where cost-efficiency is paramount and turnover is accepted to be high, unpaid are probably more common. Or when the number of applicants vastly outstrips available positions. In others, where attracting and retaining personnel is seen as a competitive advantage, payments are more likely. Even within a company there can be different divisions that do things differently. Edit: since it is is posted in computer science I should add that I have no clue how tech (i.e. non biotech) companies operate.

Chromosomes consist of two sister chromatids that get separated to the respective cells. Then, the chromatids duplicate in the S phase again.

It does not mean that they are meaningless, as they do agree with experimental outcomes. Whatever it is that allows the perpetuum mobile exist may not impact the other models in their respective domains of application. The most important bit about models is that they are useful, not necessarily that they cover every aspect.

But it isn't much different from the current state of science. Whether you look for something new or to try to refine/revise something old that does not seem to work out, it tends to take quite some effort.

AFAIK asbestos litigation started with workers being exposed to asbestos and suing their employer for compensation. I.e. harm was done by specific companies to specific targets. For litigation to environmental toxins (say, a leak in some production line), typically a causal link between the source (and hence the responsible) and the victim has to be found. I cannot see a possible way to do so for CO2, otherwise people would have been able to sue everyone else for air pollution in major US cities (before the clean air act and other measures).

What is the purpose of fundamental forces?

It appears that there is the assumption that intelligence is unique to humans. Disregarding the issue with defining intelligence for now, behavior associated with what we commonly associate with intelligence can be found in a wide range of animals to various degrees. It is rare that fundamentally unique traits, if adaptive, are not at least somewhat spread around. Exceptions tend to be adaptations to extremely unique niches or specializations (and in a way, intelligence is just the opposite of that). When the paper came fresh out I almost lost it. With one simple experiment they could have debunked it (and eventually someone else did). I use it as an example why peer-review alone is not enough. There were so many things wrong with it, starting from the composition of the group (had some famous senior authors on it, but from the wrong field, including e.g. engineers and physicists with little to now knowledge of bacterial physiology). I have been ribbing my physicist colleagues about it since then. Yeah, it seems I cannot stop ranting about it even after all that time. Also, if it was the same paper that I was thinking about it, the claim was that phosphorus could be replaced by arsenic, not carbon (the latter would require even more mental gymnastics).