

CharonY
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Everything posted by CharonY
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These cocktails are not very specific nor is there a perfect combo that completely reduces protease activity (and does not cause downstream issues). For proteomic applications we found that working quickly on ice results in little or no detectable degradation. That being said, either of the mixes would cover the range of major protease classes.
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One could also argue that terrorism is a response to someone with a much bigger stick. Trying to discourage that by wielding an even bigger stick seems like the definition of insanity.
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This is just an assertion with little evidence. In fact, many generals and other military leaders were more than willing to continue. Depending on which scholar you ask, the atomic bomb provided various degrees of leverage for the emperor to call in a surrender (and even then there were mutinies). But most would agree that it was but one of the many aspects. And some would also argue that it was completely unnecessary after the invasion of the Soviets. As others are trying to explain, these events are massively complicated and simple cause-effect type of reasoning are almost always wrong.
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In addition to what Ophiolite said, the Japanese were hoping to broker a peace with the US via the Soviets. But the invasion of Manchuria and the destruction of the largest military unit that the Japanese still had shattered that hope. Considering that the previous firebombing that occurred over Japan were far more devastating than the atomic bombs, the narrative of atomic bombs as a direct route to peace is at best misleading (and a lousy bumpersticker to boot).
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Is there anything to discuss here?
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I am not sure whether that is the intention of OP but sociopathy is generally regarded as a type of personality disorder. As is often the case, details and definitions can be fuzzy, but among the common factors named are often lack of empathy, poor impulse control and high self-confidence. The causes are under much discussion and appear to be very varied ranging from genetic influences to childhood upbringing. It will be almost impossible to elucidate defining elements. But based on that alone it is very unlikely that a singular aspect (such as games) will be the cause. In addition, one has to distinguish whether media act as triggers for sociopathic individuals in a given study/observation or whether it is a contributor in the development of psychopathy.
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Based on memory (though I could be wrong of course), in most cases the redoxpotentials would be incredibly tricky. Especially in the described scenario they would have to be electron donors but in the absence of oxygen there is little in terms of possible acceptors. Respiration-dependent accumulation was observed in some bacteria, but always in presence of oxygen. I believe there was also the possibility of iron silicates but the options are very limited. That being said, my statement does appear to be rather absolute and I should have qualified it a bit more.
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It somewhat annoys me that in these kind of reports use "feeding" for respiration as well as nutrient acquisition. Obviously, neither metals nor silicates can provide biomass. Reduced metals could be electron donors, but the question would be what the acceptor would be (respiration). And pure silicates won't do at all.
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Demanding context-free discussions pretty much precludes the educated and emphasizes the opinion part.
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Got compressed air on skin... What now?
CharonY replied to Marshalscienceguy's topic in Computer Science
I suppose you are referring to the small cans used to dust off computers and suchalike? They typically contain difluorethane (a refrigerant) rather than actually compressed. It is slightly toxic, but the label is probably just a general precaution to avoid cold damage (but you would have to dump a lot on it). But as Bignose said, it is pretty hard to harm yourself with it (except maybe trying to swallow the bottle whole). -
This discussion is pointless as it discusses not a religion per se, but followers of the religion and then even some random subset at an arbitrary point in time. As others have pointed out, there are so many different islamic countries and societies that a true discussion has to be much more in-depth and cannot be boiled down to Christianity vs Islam. Many things affecting societal values are cannot simply be reduced to one thing, even if it is as influential as religion. For example, it appears that the general conception is that Islam prevents women's rights. But look for example at voting rights. In Iran and Afghanistan they were allowed to vote in 1963, but in Switzerland it took almost a decade longer. But since then in Afghanistan things changed dramatically. So if even within a country values and rights change dramatically without a radical change in religion, how can you expect to be able to distill it down to that simple factor?
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Nor did I ever claimed it to be. However, in an earlier comment you stated: Yet an observer of that time clearly showed the opposite. While many moved for other reasons, contrary to your claim quite a number of, Palestinians who did not flee were chased out. And obviously that also means that those that have not been actively ejected, may have fled to precisely avoid that. That, in turn shows that your narrative that you are trying to spin does not conform to the actual events. It is estimated that around 80% of Palestinians left or were removed from their homes. Likewise various studies and estimates point to (near) total destruction of quite a number of Palestinian settlements, concurrent with land gain by Israel. That is the 1948 exodus, btw. The 1967 exodus has a report from the UN which describes forcible eviction following the 6-day war. The fact that Palestinians still remain does not equate the fact that they did not remove Palestinians from their home. In fact, a critical person would wonder why they became a minority in the first place. Just for perspective, Nazi-Germany had definitely an active murder program targeted at Jews (and others). However, at the end of the war there were still had Jewish survivors living in Germany (in hiding or married to non-Jews). Estimates put the survivor rate at a mere 10%. But obviously, looking back one cannot take the survivors as an example that no targeted program ever existed against Jews in Germany.
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From http://en.wikipedia.org/wiki/1948_Palestinian_exodus:
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Note that the current evidence is quite weak and likely spurious. That is not to say that there are no interactions, but they are more likely do complex metabolic interactions rather than targeted manipulations as in cases of other parasites.
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Solved is a bit strong considering that it is only a model. It is an interesting hypothesis at this point.
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Current state of protein knowledge
CharonY replied to Dr Joshua Box's topic in Biochemistry and Molecular Biology
Well 1) has not been resolved yet and is still very much an estimate. For 2+3 I would simply go to the indicated databases and perform a search and limit it to Homo sapiens as qualifier. That should give you the number based on what is in the given database. One should note that not all experimental evidence is equal. Some entries are based on sequence similarity based on evidence in other organisms (which may or may not be accurate). Or some evidence is very weak (though it would take a lot of digging to figure it out). -
Or looking back at existing data which clearly show a shift in demographics as well as causes of death. Ten years in these kind of studies are insufficient to filter out noise.
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CGI can be made very well and subtle. The good ones are those that one does not notice in the first place (often in the background, removing items/ropes etc.). However, there are far too many movies that just go overboard with because they are cheaper than real sets. A realistic skyscraper? Naah make it hundred times larger and copy/paste a gazillion of them all over the place. It is supposed to look futuristic anyway. A scary group of a dozen or so orc? Naaah, make them hundreds. What? there is not enough space, then just copy them to the walls and ceiling.
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Ebola outbreak in US...unneccssary scare?
CharonY replied to Elite Engineer's topic in Microbiology and Immunology
There are many things. Incubation period is typically not critical per se, but the thing about Ebola is that during that time people are not infectious. Spread can be facilitated if people are contagious while being presymptomatic. This is e.g. the case for many STDs but also a number of (often bacterial) infections including Bordetella pertussis. Another aspect would be slow progression, so that people do not die quickly after being symptomatic, for example. Then there is the transmission rate (which is also fairly low for Ebola) and also the specificity of symptoms. -
There are many ways to approach this. But the simplest is to make calibration curve of the peroxidase (how can you do that simply?) and then transfer that approach to an experiment where you add the immunoassay.
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I fully agree with that.
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Ebola outbreak in US...unneccssary scare?
CharonY replied to Elite Engineer's topic in Microbiology and Immunology
I think ebola is of significant local concern, but much less on a global scale. The reasons being: - it has a very aggressive progression which actually limits undetected spread - it is not that easily transmissible and require quite some extensive contact with body fluids (transient contact does poses a very low risk) On the opposite side influenza has a much higher spread, but lower fatality rate. Yet, due to the sheer amount of infections fatality estimates even in highly developed nations such as the USA have higher yearly fatalities. In other words, the very thing that currently makes Ebola so scary (high fatality rate, aggressive) is currently limiting its potential to spread. Due to its relatively low reservoir (in humans at least) there may be also a smaller potential to get nastier, but I would have to do some more reading to be able to evaluate that... -
And depending on how old they get, it may be more than one point.
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Funny bit is that the last quote given from Barfbag was based on that reference and virtually stated what I posted (repeatedly) earlier. Reading and comprehending are two different things, it seems. But I do applaud the willingness to learn about it (assuming that one really does read upon it rather than filtering websites through ones own bias). That would include informing oneself about the role of pain receptors in the Thurnberg illusion and getting a better idea what "hot" in this context actually means (and why the attempt at backpedalling does not make much physiological sense). This is indeed part of the whole system. Sensors react to stimuli but then the actual transmission circuits which ultimately determine how and which part of the brains are reached cause the actual sensation. This is why mechanisms such as the Thurnberg illusion are quite interesting as it helps deciphering low to midlevel interactions within these pathways. Identifying the actual sensors and stimuli they react to (which is often curiously unspecfic)is typically the easy part. But how they interact can be quite tricky to figure out.