CharonY

I suppose much of it is (again) due to the antipathy against Bush.

It is not the reading per se that I am worried about. Actually I wonder if one should close this thread.

I think they wanted to reach 5-7 TeV until end of this year. I am really interested when it happens. On second though I think I would be more excited once they have evaluated the data. On third thought I'd probably only be excited if they translate their findings into something that even I would understand...

I kind of have a hard time to believe that they are usable for high-end applications. I am tempted to get me a bino, but I do not think I would like to try the fluorescence system. And they spelled "Canon" wrongly on their site...

Right, I forgot about Phusion. I think I had only used it a few times for relatively small fragments (~3kb) but with high (~75%) GC. Switched to KOD as it gave me more consistent results. But having it in the freezer is usually the best reason to use it

Just to clarify, Europeans do not have a special gene allowing lactose tolerance. Every human possesses a functional lactase gene. The difference is that normally this gene will be repressed later on. However, a SNP in region near the lactase gene in Europeans allows a continuous expression of the gene). In other words, the mutation allowing lactose degradation is in fact only very small. I have to correct myself, I was reading different things but I stumbled over some recent papers on this area and it appears that there may be now altogether 5 SNPs associated with lactose tolerance. Regarding the fixation rate, the question is, of course whether the alleles may have gotten fixated in certain subpopulations first. Even if the overall population of a given area was several thousand, it is likely that exchange of genetic material was somewhat limited (though one would have to trace where precisely diary was practiced). Alternatively or additionally, of course, the selection coefficient has had to be very high. Anyway, back to topic...

While you can get those strains they are all biosafety level 2 and you need to sign a MTA. If you work in such a lab I am certain that you should know where you can get them. If you are not, you should not get them.

Unfortunately this is a very romantic view on marine biology. Not that much is actually dedicated to field work, and in the end it is mostly down to funding. Just as a side note, I am pretty sure that it wasn't meant in earnest, but I just have to stress that one shouldn't mix up Hollywood with real life research. They are simply way too far apart.

Hey wow, congrats. Does MkII has any significant upgrades?

From the top of my head, polymerases with proof reading capability and with with which I successfully amplified ~10 kb of high GC fragments (most are able to amplify up to 30kb): KOD Hot Start Qiagen LongRange Elongase (did not work that well on very high GC templates) TaqPlus Pfu Ultra LongAmp Taq There were a couple more, but these were those that I used most. BTW, 4 kb is often still within the range of most "normal" proof-reading polymerases. The yield might be a bit lower, but one could use them most of the time, too. I have successfully amplified ~4.5 kb (not high GC) with Pfx, Pfu and PWO (at least that are those that I remember).

Most of analytical chemistry would appear in the chemistry area, whereas bioanalytics tend to wind up in in the biochemistry subforum. While the distinction in disciplines might be debatable, there are usually not enough posts to to warrant a distinct subforum.

It depends on the country, a bit. In the USA grad school is, in the first years at least, still very much like school. In contrast, in Germany after the master you immediately start with a project, which then may be funded by a company. In some cases the research is even done within the company (with a university guy as supervisor). Downside is that not everything is allowed to be published, except basic research.

What precisely do you mean? Positions in the industry for PhD holders, or industry funded positions for graduate students? The latter are normally in collaboration with local universities. Also, in which country? I'll move this to science education.

Yes, you can use tags, but this does not allow a separation and maybe subsequent identification of all the proteins in crude extract (as indicated in the op). You can either capture only those that have been tagged genetically (e.g. using Hist-tags) or you would more or less randomly (in many techniques the lysine residue is targeted) label everything. This is normally not the goal if you want to analyse a crude extract.

Yes, the more mustad-like one should be the anhydrous form. I just recalled that it appears greenish under reflected light.

Also it depends whether raw performance is an issue. Even if Macs should have higher component quality, in terms of (computational) performance there are better ones for the buck.

In addition, (with the exception of E. coli you only provided the genera. There are a number of non-human pathogenous Yersinia species, for instance.

The best you could do is check the whole pathway in a standard textbook. But i am kind of surprised that you did not find good internet sources. Regarding your specific questions: how does it occur? - the specific reactions are catalysed by enzymes, as usually indicated in most depictions of the pathway (mind you, they are mostly depictions of a "standard" pathway, a number of microorganisms have modifications in that pathway) -where did the electrons go? check out the role of NAD+ -how did glucose 6 phosphate go to a 5 ring structure? glucose-6P is converted into fructose-6P, check out their structures (the empirical formulas are precisely the same, the reaction is just an isomerization) I will move this to the homework section. Your answers are more likely answered there.

Also, would you like to have a convertible laptop? I found them quite useful, so far (using it as a notepad during meetings, quick sketches and for writing down formulas or simple illustrations during lectures).

Compared to biologists.... sounds about right

Actually I am pretty sure that FeCl3 in aquous solution is reddish-brown. At least it was in my memory.

If you store it in a dry place (ideally in a air-tight container) and do not heat it up, I see no reason why it should not remain stable for an extended time. Edit: aaah, cross posted.

If you talk about sex you are automatically talking about biology. There you go

To be honest, I am kind of surprised that there is not that much Spanish around here in the south (in the labs, that is).

Mere exposure to toxic compounds is rather unlikely. Especially organic solvents can induce headaches, but even then only after lengthy exposure (and if used in larger quantities). If your headache start quickly it is quite likely the eyes, as above mentioned. Maybe you should buy your own brand new set of goggles and try them. If view is blurry it is not good to use them anyway. Do you wear glasses, btw?