Dingervb

Thanks CharonY. I went to the doc to get checked out today, and everything is filed through my employer. No eye symptoms yet, and I'll do the standard HIV, hepB, and hepC blood tests now, and in 1, 6, and 12 months. I feel like such an idiot! 12 years in human cell bio research, and you would think I would know better and not be so careless. I knew that the cells were not recently virus infected, and I think my years with primary human tissues and blood have made me lazy when it comes to caution with cell lines... The thing that bugs me now is thinking about whether any of the cells might still be in my eye or in my tear duct... Can they grow? Seems like a silly question, and I'm sure the rare possibility of infection is greater than this, but I keep seeing the ATCC web page for this line, and next to "tumor forming" it says "YES". Did they really have to put it in all caps???

Hi all, What about the risk of infection with the adenovirus5 used to transform the 293 line? Aren't all 293 lines virally transformed? And does the viral DNA retained in the genome pose any risk after exposure? I was splashed in the eye today by a very dilute droplet of 293 cells in suspension at around 150,000cells/ml. I removed my contact lens and rinsed with water immediately. Should I be concerned? These cells have not been infected with any viral vectors, but what about the adenovirus they retain? Trying not to freak out...