sunny

helo i used kamekura and onishi method for rnase activity.briefly its steps r: buffer pH 7 enzyme .1ml rna 1mg incubation for 2 hr stop solution centrifuge then in end dilution of supernatent. in many other protocol more or less all steps are same but i cant get the logic related to dilutions. is it the necessary step or what??? as if i have the O.D 2.06 of test y i dilute it???? and if i dilute at which dilution i used??? ooooh so confusing:^

can any one help me on bacterial rnase activity assay? which is the bst method for it?