Hello. i have some question regarding use of bradford method.
why is linerization of the bradford method more uncertain near 0 mg/mL concentration of protein and above 0,9 mg/mL where it bends off. In an experiment, the regressive line usually never goes by (0,0) which i assume has something to do with the uncertanty.
Using the UV - method give a more "perfect" regressive line with high precision, but if measuring contaminated protein, often gives an overestimation of the concentration, where the bradford method gives a more correct assumtion of the concentration but with lesser precision. Why is the precision lesser with bradford method?
