mohamed samir

Dear ALl, Alittle bit stricky for me to ask question like this. Iam going to perform an experiment on a co- culture consists of both human A549 pneumocyte II cells and murine macrophage J774 cell line to test the effect of miRNAs on influenza virus replication . I have no protocol for this and iam confused really whether I will be able to blend two cell line from different host. However, the media in both cells are differ , is this will pe appropriate medium for both cells ? Should I used human macrophage cell line to be compatable with the A549 cell as it is from human ? Dpepnding on which criteria, will our biculture succeed ? I can summarize the question Can I culture two differenrt cell line ( for example, A549 and J774 ) at the time both of them was cultured in different medium ? Can I culture two differenrt cell line ( for example, A549 and J774 ) that isolated from two different animal host or human ? Thanks

I usually got an melting curve with multipeak. Can anyone tell me what is the exact cause for this

Hi everybody. Iam doing isolation of miRNA from tracheal tissue of infected mice. What is bothering me really that I got surprised when I end up with low RNA yield of the sample. I red the protocol carefully and found nothing wrong with my work. Have any one knowledge what is the difference between chlroform and the chloroform /isoamyl alcohol mix. I mean the protocol stated that you have to be sure that the chloroform is not mixed with iso amyl alcohol. But I red that the iso amyl alcohol reduces the RNAase so i suppose that it will be beneficial for the isolation. I would appreciate it if any one help me.

First of all, Iam happy that you do your best to capture some valuable information from the book in your hand. Here and as far as I know that the surface of the culture bottle or the culture petri dishes characterized by presence of material, polystyrene. that help the cell to adhere to the surface and I think also when u provide the cell with nutrient and medium , after 1-2 hours it will start gowing and adhere to the surface