bekble

Hi everyone! I'd like to measure concentration of NR3C1 in sputum of asthmatics. I suppose real time PCR s the best method and becase of that I have some questions. What do I need to do this? I found primesr for this receptor. http://www.realtimeprimers.com/vhps-1818.html what else do I need to start and where can I read more about how to do this. Of course I have an access to lab but I'd like to read on my own before asking my assistant professor everything. I am a beginer in molecular technics. hope to read from you. best wishes.