Hello,
I have a question for those of you working with phages. I have isolated some temperate phages by mitomycin C induction of lysogenic bacterial strains. Now I need to amplify these phages and thus I need to find indicator strains for which the phages will be lytic and won't integrate in the bacterial genome. Accordingly, I was wondering if somebody knows why temperate phages can follow the lysogenic cycle in some bacterial strains while the same phages will be lytic and won't integrate the bacterial genome in other bacterial strains ?
Thank you!
