Hi guis, recently I ran for the first time a Western Blot + immunodetection analysis on few specific proteins and their antibodies; Beta-actine: 1°Mc Ms β-actin / 2°GAM-HRP PCNA: 1°Mc Ms PCNA / 2°GAM-HRP Occludine: 1°Pc Rb OCC / 2°GAR-biotin / 3°s-HRP I got the following results: http://s23.postimg.org/i0jea1oyz/Occludin.jpg http://s13.postimg.org/qg0r2ew2v/PCNA.jpg http://s15.postimg.org/flsazmwij/PCNA_b_actine.jpg http://s24.postimg.org/af5t0z0dx/Occludin_b_actine.jpg
Since i'm quite new to this, I really wondered what those other lines were (beside the molecular ladder). The saturated lines are the proteins (of that I'm sure), but lighter ones are unknown to me. Also I wondered wether it is possible to find a copy of the right molecular ladder somewhere online, since I lost my copy. Thank you in advance
