Thank you CharonY and Callie, I have learnt something new from you. I believe you have the right answer. I agree with Callie in that each colony is meant to have the same genome. In the process of transformation or while bacteria grow on the plate they may be forced to face different conditions that may lead to either differnt gene expressions or even point mutations. Thank you.
I wonder why bacteria form colonies in soft agar plates and why those colonies grow independently from each other. Are those colonies DNA different from each other? Thank you.
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