nazych

Hello, I have two proteins which one is wt protein, and one is mutant of the same protein. I should denature both proteins in the same conditions with 5 M urea and do NMR on both of them and they should be exactly in the same conditions. I already used protoparam tool in expasy and found the extinction coefficient of mutant and is smaller than wt, but when I denatured the protein and did NMR on them, the NMR spectrum of mutant dropped about 30%, but based on expectation, it should only drop about 15 percent. I have a problem to find their exact concentration. Can you please give me a way to calculate extinction coefficient of the mutant and wt. Thanks.

I turned it off and turned it on. It worked after that! Thanks.

Thanks.

I made collier media yesterday consisting of all amino acids and also glucose and trace element. I want do growth the day after tomorrow and I am worried about my media not getting bad, I was wondering if i can put it in cold room for the time I dont use it? Thanks.

Hi, We have an amsco lab 250 autoclave in our department. When yesterday I wanted to autocalve my flasks for growing bacteria, i saw it was saying "all utility shut down" i hit cancel on the screen and did autoclave. Today when i tried to autoclave and hit cancel it went to main menu and i choosed my option, but it did not start to autoclave and again it went to "all utility shut down" and when i hit cancel again it did not do anything and emained shut down, it seems touch screen does not do anything. Can some body help me with that? Thanks.