ymacharmcity

I appreciate the reply CharonY. When you say massive initial dilution step, were you able to still get results regularly? I've tried the mass dilutions and my results always seem to vary for FURA imaging or current clamp work.

I am trying to use beta Estradiol as a compound to test in cell culture, and it is only dissolvable in ethanol, and whenever I try to carry out serial dilutions to carry out the dilution to nanomolar levels, the hormone seems to come out of solution rapidly. Any pointers/tips/advice for a grad student in need?