ecoli

What algorithm are you using? It's a judgement call based on how many mismatches vs gaps you want in the alignment. What I would probably do is try running the algorithm over a range of gap penalties and calculating some metric (ave gaps and/or ave mismatch). Graph the metrics versus gap penalty and perhaps they'll be some inflection point where the tradeoff is acceptable. That's sort of the quick and dirty way for parameter 'tuning'

It's an xml, but I just want the text anyway so I'm good to go.

IPTG binds to and represses the lac operon repressor and does so competitively with allolactose. in high glucose, you only get low (basal) expression,with low lactose concentrations (because the repressor is off but there's no cAMP + CAP to recruit RNAPs.). IPTG has the same effect of removing the repressor, but in high glucose will only result in basal lac operon transcription. Glycerol can be converted into glucose, so it should be able to effect lac transcription, but I suppose it depends on what cells you're using (some may lack this conversion pathway).

yup, thanks. Though I'd like to save the entries first (to my harddisk or something) for my own purposes.

Also, humans have methods to police actions and enforce social norms of people we never have to personally be in contact with. Its a pretty cool effect that lets you build large civilizations, though I personally think is the most interesting on the municipal scale.

Do you have to explicitly schedule threads in Matlab?

True, but at least the ubuntu distro I'm running is not as good at energy management as runnning window's 7 on the same machine (3-4 solid hours of battery compared to ~6 when running windows). This could, theoretically, result in more thermal output.

I used to be in love with my SFN blog, but I think its time for a complete reset. I'm no longer interested in some of the things I once blogged about and somehow the comment section has exploded with spam (I had to disable notifications). I'm considering to get back into it though, and want to stay on the SFN platform. Is this doable?

And depending on how fast AI development goes, maybe not even far future.

EDTA or citrate are common anticoagulates. I'm not sure what the correct concentrations are, but I'm sure that data can be found in medical journals (a lot of studies use anticoagulents)

If you can't read the bible literally, then how do you determine if an interpretation is valid? If you can't, then any interpretation is valid and therefore the bible can't be used for any sort of reliable inferences about historical events. Which really calls into question the whole Jesus divinity thing.

would it even be possible to build a backyard fusion reactor? David Hahn's breeder fission reactor would be difficult enough, but fusion is still a huge technical challenge for the world's top engineers and scientists now.

I'm getting my PhD in a computational biology field. Many comp-bio programs accept students from related fields and you can take all the CS courses you want, if this is something you're interested in you might not need any additional formal coursework to apply to a grad school.

But you're talking about a all-powerful God who somehow fathered a son who could walk on water - surely he could've turned the world upside down to make the story consistent.

As CharonY suggested, you seem to be assuming that ribosomal RNA gets translated into protein, but it doesn't. Ribosomal RNA forms complexes with ribosomal proteins, but there's no sequence similarity (not sure about the literature on this, but I assume the prevailing hypothesis is that the protein subunits got 'added' later)

It's a trick question.. Jesus flies like a banana.

... uh oh, now you've done it.

Add some more complication by the fact that 'latino/hispanic' is not a race, in the traditional sense. Hispanics are some mixture of White, Native american and (seemingly to a lesser extent) African race - thanks to colonial patterns of interbreeding with local populations in the Americas as well as transplanted African slaves. http://blogs.discovermagazine.com/gnxp/2011/04/the-coincidental-intersection-of-sociology-and-genetics/

Having children is a personal and, for some a rewarding, choice. That being said, overriding evolution by not having children is like overriding gravity by flying in an airplane: the rules apply even if you don't realize it.

Having offspring is the evolutionary equivalent of living forever. I agree with the first part though, disagree with the second.

Generally, a poor antibody will have poor binding affinity to the target antigen or has too much cross reactivity to off-target effectors. Since the animal making the antibody is not usually the same as the model system the researcher is using the antibody in, unpredictable off-target effects are especially problematic.

Threw the table into the mirror and used the broken shards of glass to slit your wrists until freed by the sweet release of death.

There are some interesting models showing how socioeconomic variables lead to polygamy/monogamy, with foraging economics leading to the former and agriculture to the later. Modern society is more like foraging societies that have inherited farming cultural attitudes. However, how you're fitting autism into this is beyond me.

How do you know those are really women? Women do have porn, btw, they just call them [trashy] Romance Novels. There are indeed real differences in how men and women consume porn, especially judging how the vast majority of male only pornography is consumed by gay men.

If there's no profit margin, is there really a need?