oswaldonfire

Also, does anybody know if there is a limit to how much DNA can be inserted through lipofection? I am not certain, but I would think that the amount would be much higher with the liposomes than with electroporation. Thanks, Chris

Thank you, CharonY. That's pretty much what I needed to know. I knew that it was inversely related, but I wasn't sure how far I could go. However, I may try a different vector to get a larger amount of DNA into the cell. Thanks, Chris M

Hi All, Does anybody know off the top of their head what the maximum size of DNA that can be successfully electroporated is? I have been looking all over, and have not yet found a bp size that one should not go over when trying electroporation. Is it on the order of billions, millions, or thousands of base pairs? Thank you in advance, Chris M

Hi CharonY, I have not misunderstood anything. I am well aware of how particularly daunting this task is. I have intensely studied the horizontal gene transfer associated with Elysia chlorotica and it's plastid uptake process, and I am going to try to apply this in a way to my work. My particular experiment may not work, but it does happen in nature, so I know that it is possible. I asked for help regarding the species of animal cells, not for your opinion on how ridiculous this work may sound. Respectfully, Chris

Hi all, This is my first post here, and was wondering if some of you more experienced microbiologists could give me some advice. I am working along with a Professor at a University, and I am performing my own individual research. Basically the goal is to introduce Arabidopsis thaliana plastids and sections of the genome into animal cells. I do not yet know what species or type of animal cell I should use - single celled, multicellular, etc. Also, if multicellular, should I use skin cells, muscle, nerve, etc. I know that different types of cells have different reactions to foreign DNA and some uptake DNA better than others. Also, I need a cell that can also cope with additional organelles inside and adapt to the presence of the plastids. If anyone has any suggestions for me, or if you need additional information, please let me know. Any help is greatly appreciated. Thanks, Chris