Dear all,
I am a master student and i just began with siRNA transfections. I have some problems with the scramble. My scramble always give dead cells, so the results couldn't be used. Then only thing that I do differently from what i ve been demonstrated was that i place first the siRNA/scramble drop and after the siRNA buffer in the eppendorf. But that can't be the case, can be? Could you please help? I am trying to find what is going wrong. Only once it worked and i had alive cells in the scramble. I work with a 12-well plate and only mine scramble gives dead cells. Any ideas?
