pkfamily

Thank you. I will do that

How do I create the primer if I don't know what the fungal species is?

ok. How would I amplify the rRNA genes? I assume after I amplify the rRNA gene, I can insert it in a plasmid like TOPO and then send that in for sequencing.

Hey all. I'm new to this forum and I was hoping someone could help me with my mold project. My goal is to be able to grow mold I obtain from a source (rotten tomatoes or bread) and then sequence its DNA followed by a genome comparison to see what organism it is. My problem lies with the sequencing of the mold DNA. Since I obtained the isolated mold from nature, I don't know what primer sequence to create in order to sequence its DNA. Thanks.