RNA RNA EMSA

[hasmatbanu buchad]

Hello

I want details regarding RNA-RNA EMSA. I require your valuable suggestion for my problem with experiment.

I used two RNA for gel shift assay one of which is labelled with Digoxygenin. I get some shift in EMSA. I also perform the same thing with unlabelled RNA and perform EMSA assay as previous one and stained my gel with Ethidium Bromide but not able to detect any shift.

I am not able to conclude anything out of it.

Kindly suggest regarding

Waiting for your reply

Regards,

Hasmatbanu Buchad