John.matheson Posted January 11, 2017 Posted January 11, 2017 Hi All, Looking for some advice. If I have a protease liquid enzyme of unknown activity how do I calculate activity in U/ml using a colorimteric substrate? All best, Niall
ecoli Posted January 11, 2017 Posted January 11, 2017 Assuming you have an spectrophotometer, you make a linear standard with a defined product and measure optical density at the optimum wavelength.
BabcockHall Posted January 11, 2017 Posted January 11, 2017 Is this homework? Do you know what the definition of activity is?
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now