How to find protein information？

[shanglei1986]

For example, a kind of enzyme is characterized by the N-terminal Serine/Threonine kinase. And my question is how to find the kinase activity site, how to make the kinase dead with one site mutation without any reference.

[CharonY]

There are several databases that can assist you in that, though of course without actual experimental verification they all will just be predictions based on sequence similarities. Uniprot is a general database that aggregates a lot of this information. If you want to implement something yourself you can look into protein family/domain databases such as pfam, prosite, etc.

But again, usually you would knock out the whole domain, or at least a sizeable chunk of it. Figuring out the required minimum mutation to deactivate a given activity is quite a bit trickier.

[shanglei1986]

There are several databases that can assist you in that, though of course without actual experimental verification they all will just be predictions based on sequence similarities. Uniprot is a general database that aggregates a lot of this information. If you want to implement something yourself you can look into protein family/domain databases such as pfam, prosite, etc.

But again, usually you would knock out the whole domain, or at least a sizeable chunk of it. Figuring out the required minimum mutation to deactivate a given activity is quite a bit trickier.

Thanks for your reply