Correcting for dilution- spectrophotometer

[northernlad2690]

i wonder if anyone could offer some subtle insight,

 

I have a set of standards of substance X with an appropriate standard curve constructed from this info. The sample of unknown concentration is diluted 10X and thus has produced a much smaller absorbance reading as expected, but I'm stuck on how to correct for this dilution as i'm not sure whether to;

 

A) multiply the absorbance reading by 10 or

B) multiply by 10 the figure from the point on the graph where the diluted absorbance reading is.

[CharonY]

You read the concentration in your test vial (the diluted sample) directly from the calibration curve. Then you multiply the result. The reason is that instruments (and hence, created standard curves) have a certain dynamic range (and may not be perfectly linear).

 

Also, if your measurement of your diluted sample falls outside of the range, you have to adjust concentration.