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Posted

Hello everyone! I'm a new member and this is my first time posting here for help.

 

I have an assignment where I have been given MALDI-TOF data for an apomyoglobin standard and a thrombin digested GST tagged of an unknown protein. I am having trouble identifying the peak indicated with a question mark in the standard data. So far, I have identified the middle peak in the thrombin digested protein as GST, while the first peak (11499.1) as the unknown, which I would be analysing with bioinformatic methods. I would really appreciate it if someone could help me or rather give me information as to how to identify the remaining peaks in both datas. THANK YOU for reading!

post-128222-0-31084100-1491642800_thumb.png

  • 11 months later...
Posted

image.png.f6408310479464eb2abdceb50a961281.png Lest consider it without the middle right Heme, your data are 100% sure?

I took a look, and it said that technology break the large into not so small part ? I need more Info? 

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