girl322 Posted June 6, 2017 Share Posted June 6, 2017 Hi, I need a proper direction. I am conducting an independent experiment. The aim is to put an ectA gene into the yeast. It seems that I cannot get an ectABC operon from anywhere, however SigmaAldrich has ectoine for sale. So, I am having a protein - but I need to get a gene of interest. In the terms of experiment, how do I go from there? Do I need to construct a cDNA? Regards, I. Link to comment Share on other sites More sharing options...
hypervalent_iodine Posted June 7, 2017 Share Posted June 7, 2017 You can't get it made? I had thought there were companies that did this, though I couldn't tell you which ones. Link to comment Share on other sites More sharing options...
girl322 Posted June 8, 2017 Author Share Posted June 8, 2017 I've tried to find it anywhere- but I can't. The companies sell only the protein - ectoine. But I am interested in the particular gene - ectA gene. So if I have a protein, do I create a cDNA library and then use primers to find a specific gene of interest? Link to comment Share on other sites More sharing options...
hypervalent_iodine Posted June 8, 2017 Share Posted June 8, 2017 There are companies that sell custom genes, like Genscript or Biomatik. Have you tried them? Link to comment Share on other sites More sharing options...
CharonY Posted June 8, 2017 Share Posted June 8, 2017 Alternatively, if you know from which organism you want it, just get it (or its DNA) from a culture bank, get the right primers and amplify it. Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now