transforming an yeast with the ectA gene

[girl322]

Hi,

 

I need a proper direction.

I am conducting an independent experiment.

The aim is to put an ectA gene into the yeast.

It seems that I cannot get an ectABC operon from anywhere, however SigmaAldrich has ectoine for sale.

 

So,

 

I am having a protein - but I need to get a gene of interest.

In the terms of experiment, how do I go from there?

 

Do I need to construct a cDNA?

 

Regards,

I.

[hypervalent_iodine]

You can't get it made? I had thought there were companies that did this, though I couldn't tell you which ones.

[girl322]

I've tried to find it anywhere- but I can't. The companies sell only the protein - ectoine. But I am interested in the particular gene - ectA gene.

 

So if I have a protein, do I create a cDNA library and then use primers to find a specific gene of interest?

[hypervalent_iodine]

There are companies that sell custom genes, like Genscript or Biomatik. Have you tried them?

[CharonY]

Alternatively, if you know from which organism you want it, just get it (or its DNA) from a culture bank, get the right primers and amplify it.