Baacteria produce defective protein, why?

[tonyb121985]

A human gene for protein hormone has been isolated. Scientist want to introduce gene into a bacterial host to modify the bacteria so it can produce this hormone. After successfully introducing the gene on a plasmid, the bacteria produced the protein hormone but the protein was defective, longer than normal, with intervening stretches of amino acid not found in the native protein. Which solution would be most likely to resolve this problem. What is most likely the explanation?

a.       Bacteria cant transcribe the human gene

b.       The bacterial ribosomes make errors when transcribing human mRNAs

c.       The gene that was introduced contained introns

d.       Bacterial cells cannot translate mRNAs transcribed from human genes

(from my research I think C is the answer)

 

Now, what is most likely a solution to resolve this problem?

A.      Start over, introducing complementary DNA (cDNA) copy of the gene into bacterial host

B.      Introduce human mRNA into bacterial host

C.       Introduce human ribosomes into bacteria

D.      Treat defective protein with enzymes